| LR-MDS (n = 14) | Controls (n = 14) |
---|
Neutrophil immunophenotypic score 2 (0–8) |
Score | 2 (0–3) | 0 (0–0) |
Score rank (Σ) | 28 | 0 |
Score classes |
0 (no dysplasia) | 1 (7%) | 14 (100%) |
1 (possible dysplasia) | 2 (14%) | 0 (0%) |
2–3 (mild dysplasia) | 11 (79%) | 0 (0%) |
4–6 (moderate dysplasia) | 0 (0%) | 0 (0%) |
7–8 (severe dysplasia) | 0 (0%) | 0 (0%) |
Monocyte immunophenotypic score 2 (0–2) |
Score | 1 (0–2) | 0 (0–0) |
Score rank (Σ) | 12 | 1 |
Score classes |
0 (no dysplasia) | 5 (36%) | 14 (100%) |
1 (probable dysplasia) | 6 (43%) | 0 (0%) |
2 (dysplasia) | 3 (21%) | 0 (0%) |
Myeloid immunophenotypic score 2 (0–10) |
Score | 3 (0–5) | 0 (0–1) |
Score rank (Σ) | 39 | 1 |
Score classes |
0 (no dysplasia) | 1 (7%) | 14 (0%) |
1 (possible dysplasia) | 1 (7%) | 0 (0%) |
2–4 (mild dysplasia) | 11 (79%) | 0 (0%) |
5–7 (moderate dysplasia) | 1 (7%) | 0 (0%) |
8–10 (severe dysplasia) | 0 (0%) | 0 (0%) |
- Abbreviations: LR-MDS lower risk myelodysplastic syndromes, PB peripheral blood
- Results are expressed as absolute and (relative) frequencies or as median (range) values
- Percentages were approximated to the closest full unit
- Neutrophil score: The following parameters were considered: FSC, SSC, CD10, CD11b, CD11c, CD13, CD16, and CD45. Values within the mean ± SD or above the mean + 2SD were scored with “0”. Abnormally low parameter (< mean-2D of the values observed in controls) were scored as follows: (< mean-2D: 0.25 points; < mean-3SD: 0.5 points; < mean-3SD: 1 point
- Monocyte score: The following parameters were considered: % of CD16+ monocytes and % of CD56+ monocytes. These parameters were scored as follows: CD14 + CD56+ monocytes: ≤18% (0 points); >mean + 2SD (18%): 0.25 points; > mean + 3SD (23%): 0.5 points; > mean + 4SD (28%): 1 point. CD14 + CD16+ monocytes: ≥5%: 0 points; CD14 + CD16+ monocytes < 5%: 0.25 point; CD14 + CD16+ monocytes: < 2.5%: 0.5 points; CD14 + CD16+ monocytes: < 1.25%: 1 point
- Myeloid score: obtained by adding up the neutrophil score 1 and the monocytic score achieved for each patient
- The following score rankings were obtained for each of the parameters analyzed: Patients: FSC = 6.5; CD11c = 5.5; CD11b = 5.0; SSC = 4.5; CD10 = 2.5; CD16 = 2; CD13 = 1.0; CD15 = 0.0; CD45 = 0.5. Controls: CD15 = 0.25; CD45 = 0.25; other parameters = 0